Tohoku Univ. Technology : Quantitative analysis of amyloid nucleation from droplets : T21-234
Applicable to the screening of amyloid nucleation inhibitor
Recently, amyloid fibrils, which relate to various diseases (Alzheimer’s diseases, ALS, etc.), is considered to generate in liquid-like droplets of amyloid precursor proteins (APP). The quantitative and high-throughput analysis of the amyloid nucleation from the droplets is important for finding inhibitors of amyloid formation. However, there was only qualitative discussion on nucleation process from droplets so far. This invention provides the method of calculating the quantitative nucleation rate J in a short time by a fluorescence dye and statistical image analysis. The thermodynamic parameter J makes it possible to evaluate amyloid nucleation inhibition capacity of drug candidates regardless of the experimental system, so, this indicator is superior to conventional indicator (lag time).
- Company：Tohoku Techno Arch Co., Ltd.
- Price：Other
